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Toronto REALTOR® Sam Kamra Launches RealEstateBuyer.ca to Buy Homes Directly for Cash Across Ontario and Alberta
Toronto, ONIf you’ve taken even a casual glance at local real estate headlines lately, you already know the story: soaring holding costs, unpredictable interest rate shifts, longer days on market, and buyers taking their sweet time to make an offer. For homeowners across the Greater Toronto Area and major urban hubs in Alberta, selling a home […]
Toronto, ON
If you’ve taken even a casual glance at local real estate headlines lately, you already know the story: soaring holding costs, unpredictable interest rate shifts, longer days on market, and buyers taking their sweet time to make an offer. For homeowners across the Greater Toronto Area and major urban hubs in Alberta, selling a home through traditional channels can feel like an exhausting test of endurance.

Between cleaning up for last-minute open houses, staging every room, dealing with conditional offers that fall apart at the eleven hour, and watching mortgage renewal dates loom on the horizon, the pressure on property owners has never been higher.
To solve this friction, veteran Toronto REALTOR® Sam Kamra—a top-producing Sales Representative with RE/MAX Millennium Real Estate who has closed over $1 billion in career sales volume—has officially launched RealEstateBuyer.ca.
Co-founded alongside software architect and technology lead Alex Prikhodko, RealEstateBuyer.ca operates as a dedicated direct-purchase platform. It gives property owners across Ontario and Alberta a fast, guaranteed, and off-market path to liquidate property or execute strategic joint-venture renovations without paying real estate agent commissions, hosting public open houses, or waiting months for conditional financing to clear.
The platform’s rollout has already captured major media attention, including a featured story in The Globe and Mail coverage on RealEstateBuyer.ca. For a comprehensive look at the regional rollout, read the full Sam Kamra RealEstateBuyer.ca release announcement.
Navigating the Shift in Canadian Real Estate
To understand why direct buying platforms are surging in popularity, you have to look at what’s actually happening on the ground in real estate markets across Canada.
For years, the standard playbook was simple: put a sign in the front yard, list on the public MLS® system, host a weekend open house, and wait for multiple offers to come rolling in. But today’s economic climate has created a very different reality for sellers:
- Stricter Lender Underwriting: Traditional buyers are finding it harder than ever to secure final mortgage approvals, causing conditional deals to collapse just days before closing.
- Escalating Holding Costs: Property taxes, maintenance, utility bills, and high interest payments mean that every extra month a home sits listed eats directly into a homeowner’s hard-earned equity.
- Private Mortgage Renewal Stress: A massive wave of short-term private loans and secondary mortgages are coming due. Homeowners facing sky-high renewal rates or sudden calls for capital often don’t have 90 to 120 days to wait for a traditional buyer.
- Estate & Probate Complexity: Inheriting a property that requires major structural work or clearing out decades of belongings can quickly become a multi-month burden for estate executors and families.
While direct buying provides a lifeline for sellers needing speed and certainty, the direct-buying industry itself has developed a serious image problem—one that Sam Kamra is actively working to correct.
Direct Balance-Sheet Buying vs. Unlicensed Assignment Wholesalers
One of the primary drivers behind launching RealEstateBuyer.ca was educating consumers on the stark difference between genuine balance-sheet investors and the influx of speculative “assignment wholesalers” operating throughout Ontario and Western Canada.
If you’ve ever seen hand-written “We Buy Houses for Cash” flyers taped to hydro poles or posted on social media boards, you’ve seen wholesaler marketing in action. The reality behind most of these operators is concerning:
How Wholesaler Assignment Schemes Really Work
- Low-Ball Option Contracts: Wholesalers target stressed property owners, getting them to sign a purchase contract far below market value by promising a quick, hassle-free cash purchase.
- The Middleman Shopping Spree: The wholesaler doesn’t actually have the cash or capital to buy the property themselves. Instead, they take the purchase contract and shop it around to private flippers or speculative buyers, charging a massive “assignment fee” (often $30,000 to $100,000+) just for handing over the paperwork.
- Double-Dipping Equity Loss: Because both the middleman wholesaler and the end investor need to make a substantial profit, the seller gets squeezed from both sides, receiving a fraction of what their home is actually worth.
- High Risk of Abandoned Deals: If the wholesaler fails to find a secondary buyer willing to pay their assignment markup before the closing date, they simply walk away using contract loopholes. The seller is left high and dry, having wasted precious weeks while carrying ongoing mortgage debts.
The RealEstateBuyer.ca Approach
In sharp contrast to speculative assignors, RealEstateBuyer.ca acts as the actual principal buyer. Engineered by tech lead Alex Prikhodko and backed by private balance-sheet capital, Sam Kamra evaluates each property directly.
- Zero Assignment Surcharges: You deal directly with the end buyer, meaning no predatory middlemen taking cuts out of your equity.
- Firm, Unconditional Contracts: Agreements are executed without subject-to-financing or conditional inspection clauses designed to renegotiate the price down later.
- Direct Private Capital: Purchases are funded directly through verified balance-sheet reserves, guaranteeing that when a closing date is set, the funds arrive at your lawyer’s office on time.
The Joint Venture (JV) Renovation Model: Unlocking Maximum Equity
A direct cash buyout is a fast, unconditional solution, but it isn’t the only way to sell. Many homeowners know their property could command a top-tier price if it were updated, but they simply lack the $50,000 to $150,000 in cash required for a modern renovation—or they don’t want the headache of managing general contractors, architectural plans, and city building permits.
To solve this, RealEstateBuyer.ca offers a structured Joint Venture (JV) Renovation Framework. This option lets property owners capture the post-renovation upside of their home without spending a single dollar out-of-pocket or taking on construction risks.
How the Joint Venture Renovation Framework Works:
- Title Security: The homeowner remains 100% on legal title throughout the entire project. You never sign away your property rights.
- 100% Capital Injection: Sam Kamra and his venture capital pool provide 100% of the funds needed for materials, labor, staging, permits, and design work.
- Turnkey Management: The project management team handles the entire process from start to finish—including architectural drawings, municipal building permits, hiring licensed trades, interior design, and professional staging.
- Public Market Exposure: Once transformed into a move-in ready showcase home, the property is listed on the open market to attract top-dollar bids.
- Profit Distribution: When the sale closes, the homeowner receives their guaranteed base equity plus an agreed-upon share of the additional profit generated by the renovation.
Expanding into Alberta: Calgary, Edmonton, and Beyond
While Ontario remains a core market, RealEstateBuyer.ca has officially expanded its direct purchasing operations into Alberta, targeting key growth centers like Calgary, Edmonton, Red Deer, and surrounding municipal corridors.
Alberta’s real estate dynamics are moving at record speed. Driven by inter-provincial migration, strong economic fundamentals, and an influx of buyers relocating from higher-cost coastal provinces, Western Canadian real estate demands agile solutions.
Core Regional Operations:
- Ontario Regions: Toronto & Core GTA, York Region (Vaughan, Markham, Richmond Hill), Peel Region (Brampton, Mississauga), Durham & Halton Regions, Hamilton & Niagara Corridor.
- Alberta Expansion: Greater Calgary Area, Edmonton Metropolitan Region, Red Deer & Central Alberta, Airdrie, Cochrane, Okotoks, and high-growth suburban hubs.
Why Expanding Direct Cash Purchases to Alberta Matters
- Fast-Moving Inter-Provincial Sellers: Homeowners relocating between Ontario and Alberta often need synchronized closing dates so they aren’t stuck paying two mortgages simultaneously.
- Out-of-Province Estate Holdings: Ontarians who inherit properties in Calgary or Edmonton frequently prefer a direct, as-is cash sale over traveling out west to manage repairs and showings.
- Investor Portfolio Restructuring: Real estate investors rebalancing portfolios between Western Canada and Ontario can liquidate single properties or entire multi-unit packages quickly without tenant eviction friction.
Clearing Up the Corporate Structure: Independent Entities & Key Roles
To ensure complete transparency, regulatory compliance, and market clarity, Sam Kamra maintains clear boundaries across his professional licensed practice, independent tech business ventures, and mortgage broker relationships.
1. RE/MAX Millennium Real Estate
Sam Kamra conducts his licensed real estate representation for traditional buyers and sellers as a Sales Representative with RE/MAX Millennium Real Estate, led by Broker of Record Ron Sally.
- Important Distinction: RE/MAX Millennium Real Estate and Broker of Record Ron Sally represent the brokerage under which Sam Kamra conducts licensed real estate agency activities. Neither RE/MAX Millennium nor Ron Sally are parent corporations, co-owners, or financial partners of RealEstateBuyer.ca.
2. RealEstateBuyer.ca Direct Purchase Platform
RealEstateBuyer.ca is a private, independent business venture owned and operated directly by Sam Kamraand tech co-founder Alex Prikhodko. It functions as a private acquisition vehicle for off-market cash purchases and Joint Venture renovation projects across Ontario and Alberta.
3. LendingHub.ca Mortgage Brokerage
For homeowners, buyers, or real estate investors seeking traditional mortgage financing, debt refinancing, or private lending arrangements, services are provided by independent mortgage brokerages such as LendingHub.ca, founded by Principal Broker Sunil Sally.
- Important Distinction: LendingHub.ca is an independent mortgage brokerage serving clients across numerous real estate firms in Ontario. It operates as an unaffiliated entity from RealEstateBuyer.ca.
Exposing Industry Misconduct: Fraud, Inflated Appraisals & Predatory Loans
Beyond building technology and executing real estate transactions, Sam Kamra is a vocal advocate for consumer protection and market integrity.
In a widely discussed national report, Sam Kamra exposed rampant fraud in the mortgage and real estate industries on Financial Post. The expose shed light on systemic issues that have harmed everyday homeowners and destabilized local property values.
Key Issues Exposed in the Financial Post Feature:
- Fabricated Income Documentation: Unethical bad actors forging job letters and bank statements to trick lenders into approving unmanageable debt loads.
- Manipulated Property Appraisals: Artificially inflating comparative home evaluations to justify oversized private loans with predatory fee structures.
- Predatory High-Fee Private Mortgages: Short-term loans stacked with upfront broker charges, lender fees, and high interest rates designed to push financially stressed homeowners into default.
- Assignment Contract Exploitation: Middleman wholesalers using aggressive tactics to lock vulnerable seniors or estate executors into predatory contracts.
“Consumer trust is the backbone of the housing market,” states Sam Kamra. “Whether a homeowner decides to list traditionally through RE/MAX Millennium, secure mortgage financing via an independent brokerage like LendingHub.ca, or take a direct off-market cash offer on RealEstateBuyer.ca, they deserve total legal transparency, zero hidden fees, and absolute honesty.”
Buying “As-Is”: What Types of Properties Qualify?
One of the biggest pain points when selling a home on the open market is prepping the property for picky buyers. Repairs, staging, deep cleaning, and tenant management can cost tens of thousands of dollars before a single offer comes in.
RealEstateBuyer.ca purchases residential assets strictly
Media Contact Details
Sam Kamra
Email: Send Email
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Nonprofit Organization, Citizens Academy US, Makes Official Public Release of Guide to Complete Understanding of the Preamble to the U.S. Constitution
Washington, DCCitizens Academy U.S., a nonprofit dedicated to helping Americans understand the U.S. Constitution’s Preamble, has officially released The Guide to Complete Understanding of the Preamble to the United States Constitution in celebration of Constitution Day, September 17. The organization is hosting a free online event, “The Day ‘We the People’ Appeared,” on September 13, 2026, […]
Washington, DC
Citizens Academy U.S., a nonprofit dedicated to helping Americans understand the U.S. Constitution’s Preamble, has officially released The Guide to Complete Understanding of the Preamble to the United States Constitution in celebration of Constitution Day, September 17. The organization is hosting a free online event, “The Day ‘We the People’ Appeared,” on September 13, 2026, exploring the Preamble and its six purposes.
The Preamble Guide Simplifies Understanding of the Six Purposes of the U.S. Constitution
In celebration of Constitution Day, September 17th, the Citizens Academy U.S., a nonprofit organization with the goal to help hundreds of millions of Americans understand the Preamble to the U.S. Constitution, just made their official public release of The Guide to Complete Understanding of the Preamble to the United States Constitution.

As noted on their website, the Preamble lays out all six purposes of the U.S. Constitution, and they are working to restore clarity and shared understanding of the Preamble and thus these six purposes. They do not explain or interpret what it means, they provide an enjoyable and easy way to completely understand it, so people can make up their own mind as to what it means.
Constitution Day is celebrated annually across the U.S. every September 17th as it is the day when 39 delegates signed the United States Constitution at Independence Hall in Philadelphia in 1787. In addition to the official release of their Guide, Citizens Academy US is hosting a free online event on Sunday, September 13, 2026, at 11am PT/2pm ET, entitled, The Day “We the People” Appeared. The event is expected to run for 1.5 hours and is open to free public registration at this link.
Academy officials have observed that many Americans who have attempted to read the Constitution have difficulty in understanding it. They attribute this to the fact that languages change over time, and many words do not mean what they meant when the Constitution was drafted. “Definitions from the late 1700s vary drastically from today’s definitions,” said Academy spokesperson Steven Ferry, “and it is vitally important that the Constitution is read with reference to the original definitions in use at the time it was written.”
To help people overcome this challenge, Citizens Academy US provides definitions in their Guide based on those from Noah Webster’s 1828 American Dictionary of the English Language. This early dictionary reveals what the words meant to the Founders and the people at the time the Constitution was written.
As an example, the definition of “welfare” in the Guide, based on Webster’s 1828 dictionary is: “the enjoyment of peace and prosperity, and other benefits of society, such as protection of rights, public safety, justice, and liberty.” Today’s Oxford Learner’s Dictionary includes an additional definition which people often refer to: “practical or financial help that is provided, often by the government, for people or animals that need it.”
Academy officials have also observed that when the Preamble is read with the original definitions in mind, understanding the six purposes of the Constitution becomes simple and the results are profound. The principle that forms the basis of the Guide is a quote by best-selling author L. Ron Hubbard, who wrote, “Complete understanding depends upon freedom from misunderstood words.”
By visiting the Citizens Academy US website, you can view the Guide and download it for free or purchase the hardcover or softcover in their bookstore.

In honor of Constitution Day, Citizens Academy US is hosting a free online event, titled “The Day ‘We the People’ Appeared” on September 13, 2026, at 11am PT/2pm ET
The Citizens Academy US is a fully IRS recognized 501 (c)(3) charitable organization with the mission to bring about a greater understanding in America. The goal of the Citizens Academy US is to have 100’s of millions of Americans understand the first sentence of the United States Constitution, which is called the Preamble. The Preamble contains the six purposes of the Constitution and understanding the Preamble enables Americans to grasp what the Constitution is meant to achieve.
For more information about Citizens Academy US, please visit: www.CitizensAcademy.us
Media Contact Details
Steven Ferry
Citizens Academy US
Email: Send Email
Phone: 727-492-4082
Website: citizensacademy.us
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Ángela Rodríguez Expands REFLEJO™ Method and Inclusive Beauty Education Initiatives
NEW YORK, USAAuthor, Beauty Educator, and founder of Divina Makeup advances international education, accessibility, and community-focused programs centered on makeup, autonomy, and women’s empowerment.
Author, Beauty Educator, and founder of Divina Makeup advances international education, accessibility, and community-focused programs centered on makeup, autonomy, and women’s empowerment.
NEW YORK, USA
Colombian-born author, Beauty Educator, and entrepreneur Ángela Rodríguez is expanding her work around the REFLEJO™ Method, an educational self-makeup methodology designed to help women better understand their individual features, needs, and personal approach to beauty.

Ángela, founder of Divina Makeup and author of Maquíllate en 6 Pasos (Make Yourself Up in 6 Steps), is developing the next stage of her educational platform through an updated edition of her book, digital learning resources, accessibility initiatives, international outreach, and community programs focused on women’s confidence, autonomy, and inclusion.
At the center of the initiative is the REFLEJO™ Method, which is based on the principle that every woman is unique and should learn to understand her own face rather than simply reproduce makeup techniques created for someone else.
For Ángela, makeup education extends beyond teaching the application of foundation, concealer, or eyeshadow. Her approach focuses on providing women with practical knowledge that can help them make informed decisions about their appearance while developing greater confidence in their own skills.
“True transformation begins when a woman learns to see herself differently,” Ángela said.
Expanding the REFLEJO™ Method
Ángela is currently developing a new edition of Maquíllate en 6 Pasos that will incorporate the REFLEJO™ Method and expand the educational resources available to readers.
The new edition is planned to include QR codes connecting readers with complementary audiovisual learning materials. The project is also being developed with accessibility resources intended to make makeup education more inclusive for people with hearing disabilities, including sign language interpretation and subtitled content.
The initiative reflects Ángela’s broader objective of making beauty education more accessible to women with different backgrounds, ages, cultures, and life experiences.
She also intends to expand the REFLEJO™ Method internationally as an educational framework that can be used across workshops, community programs, and other learning environments.
From New York Fashion Platforms to Education
Since arriving in New York in 2020, Ángela has worked across several areas of the beauty industry while establishing herself as an educator, author, professional makeup artist, and entrepreneur.

Her professional experience has included participation in major New York fashion platforms, including New York Fashion Week, where she has held leadership roles within makeup teams.
Alongside her work as a makeup artist, Ángela has focused on creating professional development opportunities for emerging artists. She has supported initiatives that allow aspiring makeup professionals to gain experience at fashion shows, develop portfolios, strengthen practical skills, and gain exposure to professional working environments.
Her makeup artistry has also appeared in publications and magazine covers connected with New York and France.
As an educator and entrepreneur, Ángela has participated in events throughout the Latino community in the United States, using those opportunities to discuss makeup education, entrepreneurship, confidence, and professional development.
Bogotá International Book Fair Milestone
In 2026, Ángela participated in the Bogotá International Book Fair (FILBo), where she presented her work as an author and discussed her approach to connecting makeup education with women’s empowerment.
The appearance represented another step in Ángela’s effort to expand her work beyond traditional beauty services and develop a broader educational platform.
Her current work builds on that experience by integrating her published material with the REFLEJO™ Method, digital education, accessibility, and community engagement.
Bringing Beauty Education to Communities
Ángela is also strengthening partnerships and developing social initiatives aimed at providing free workshops focused on self-makeup, self-esteem, and empowerment.
The planned initiatives are intended to reach women in Afro-descendant, rural, and Indigenous communities in Colombia, as well as other populations that may have limited access to beauty education and related professional-development opportunities.
Through these programs, Ángela aims to apply the experience she has developed in the beauty industry to educational and community-based settings.
She has also begun collaborating with organizations and foundations that support women while developing a broader social-impact platform centered on education, leadership, inclusion, and dignity.
Connecting Beauty Education With Women’s Rights
Ángela recently participated in an event held within the context of the United Nations focused on education for peace and human rights.
She describes the experience as an important development in her work and one that strengthened her interest in participating in initiatives involving women’s rights, education, dignity, and access to opportunity.
Her long-term vision is to connect beauty education with wider social-impact initiatives and establish strategic partnerships capable of bringing those programs to additional communities.
For Ángela, women’s empowerment encompasses more than personal appearance. Her work increasingly addresses education, autonomy, inclusion, professional opportunity, dignity, and women’s ability to make informed decisions about their own lives.
An Immigrant Entrepreneur Building From New York
Ángela’s professional development also reflects her experience as a Colombian immigrant establishing a career in the United States.
Since moving to New York in 2020, she has worked to build her professional identity across makeup artistry, education, publishing, and entrepreneurship while developing Divina Makeup and the REFLEJO™ Method.
Her focus has gradually shifted from applying makeup for clients toward teaching women how to understand their individual features, develop practical skills, and approach their personal image with greater independence.
Through fashion, education, publishing, community initiatives, and international outreach, Ángela is continuing to develop a platform that brings together beauty education and social purpose.
About Ángela Rodríguez
Ángela Rodríguez is a Colombian-born author, professional makeup artist, Beauty Educator, entrepreneur, and creator of the REFLEJO™ Method. She is the founder of Divina Makeup and author of Maquíllate en 6 Pasos. Based in New York, her work focuses on self-makeup education, professional development, inclusion, accessibility, women’s empowerment, and community-focused initiatives.
Instagram: @divina.make.up
Media Contact
Ángela Rodríguez
Author | Beauty Educator | Creator of the REFLEJO™ Method
Founder, Divina Makeup
New York, United States
Instagram: @divina.make.up
Photo Credit: Photos accompanying this release were taken by New York-based photographer Dong Yang.
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BTI, Cornell Develop AI Tool to Map Hidden Universe of Small Molecules
Ithaca, NYResearchers at the Boyce Thompson Institute and Cornell University have developed AIMe (AI Molecule Explorer), a neuro-symbolic AI tool that predicts, organizes, and searches mass spectra for more than 100 million known small organic molecules—creating a searchable map of chemical space roughly a thousand times larger than existing experimental libraries. Applying AIMe to gut microbiome data, the team identified a previously unknown, ring-shaped polyamine compound also found in human fecal samples, and used the tool to annotate millions of unidentified spectra in a major public mass spectrometry database.
Researchers at the Boyce Thompson Institute and Cornell University have developed AIMe (AI Molecule Explorer), a neuro-symbolic AI tool that predicts, organizes, and searches mass spectra for more than 100 million known small organic molecules—creating a searchable map of chemical space roughly a thousand times larger than existing experimental libraries. Applying AIMe to gut microbiome data, the team identified a previously unknown, ring-shaped polyamine compound also found in human fecal samples, and used the tool to annotate millions of unidentified spectra in a major public mass spectrometry database.
Ithaca, NY
Researchers at the Boyce Thompson Institute (BTI) and Cornell University unveiled AIMe (AI Molecule Explorer), a neuro-symbolic artificial intelligence tool that predicts and maps the mass spectra of more than 100 million known small organic molecules. The tool tackles a long-standing bottleneck in biomedical science—more than 80% of small molecules detected in a typical biological sample cannot be matched to a known structure—a gap that has stalled discoveries linking the gut microbiome, immunity, and metabolism.

The work is a collaboration between Frank Schroeder, Professor at BTI and in Cornell’s Department of Chemistry and Chemical Biology, and Carla Gomes, Professor of Computing and Information Science and director of Cornell’s AI for Science Institute.
A different approach to an old problem
Mass spectrometry is the workhorse for small molecule identification used in a wide range of applications from toxicology to food analysis. When a compound is analyzed, the instrument fragments it and records the masses of the resulting pieces. That pattern of fragments, the tandem mass spectrum (or MS2 spectrum), functions as a molecular fingerprint. To identify an unknown compound, researchers compare its spectrum against a reference library of spectra from known compounds or develop hypotheses as to what the structure of a compound may be based on manual analysis of the fragmentation pattern.
The problem is that experimental reference libraries remain sparse, while expert, one-by-one interpretation is labor-intensive and slow. Collectively, available libraries cover fewer than 1% of known compounds, and resolving the structure of a single unknown can take days to months of iterative analysis and experimental validation. As a result, spectra without close library matches usually remain unannotated.
AIMe takes a different approach. Rather than waiting for experimental spectra to accumulate in libraries, it predicts spectra computationally—then organizes those predictions into a searchable resource called MS2KOSMOS. AIMe generated more than 800 million predicted spectra covering essentially all known small organic molecules in PubChem, the largest publicly available chemical database. That represents roughly a thousandfold expansion of searchable chemical space relative to existing experimental libraries.
“At the core of AIMe is DeepMS2Reasoner, a model that simulates how molecules fragment inside a mass spectrometer,” explained Gomes. “It builds fragmentation pathways step by step, using symbolic chemical rules to enumerate physically plausible fragmentation steps and a neural network to assign likelihoods to each step. The result is a predicted spectrum and an annotated map of how a molecule came apart—a feature that makes AIMe’s outputs interpretable in chemical terms, not just computationally useful.”
From mouse gut to human biology
To demonstrate what AIMe can do in practice, the researchers applied it to a comparative metabolomics dataset from mice. The experiment compared germ-free mice, animals raised without any gut microbiota, against mice with a normal complement of gut bacteria. Several thousand chemical features differed between the two groups, and most of the abundant ones could not be identified using standard methods.
The team used AIMe to query MS2KOSMOS with spectra from the 111 most abundant unidentified microbiota-dependent compounds. For roughly a third, AIMe retrieved close predicted spectral matches and related structural candidates, providing chemically interpretable leads for follow-up. For the rest, AIMe mapped the unknown spectra to molecular neighborhoods—sets of structurally related compounds whose shared fragmentation patterns could inform hypotheses about what the unknowns might be.
“Two compounds in particular became a case study in what AI-guided structure elucidation can accomplish,” said Schroeder. “Both produced spectra that suggested they were polyamine derivatives, a well-studied class of molecules, but the fragment patterns didn’t match anything previously described. Using AIMe’s output as a guide, our team assembled candidate structures combinatorially, predicted spectra for each candidate, and used the comparison to narrow the field.”
For the first compound, the best candidate was a linear putrescine derivative, which was then easily verified by synthesizing an authentic standard. For the second, the predicted spectra of potential candidates consistently failed to explain two prominent peaks—until the team expanded the search to include cyclized variants.
Synthesis confirmed what the predictions suggested. The second compound turned out to be a structurally unusual macrocyclic polyamine—a ring-shaped variant unlike any previously reported from mouse or human biology. Searches of a large public mass spectrometry database subsequently found the same compound in samples of human origin, detected in 57 of 99 human fecal samples examined.
Polyamines occupy an important position in biology. They sit at the intersection of diet, the gut microbiome, and immune function—and these findings suggest that the catalog of microbiota-dependent polyamines is far less complete than scientists assumed.
Annotation at scale
The researchers also tested AIMe at repository scale, applying it to more than 7 million spectral clusters from the Global Natural Products Social Molecular Networking database, one of the largest publicly available repositories of mass spectrometry data. Prior annotation efforts had annotated roughly 416,000 of those clusters. AIMe returned putative annotations for approximately 2.69 million, using the same similarity threshold applied in that earlier effort.
AIMe is accessible at https://www.cs.cornell.edu/gomes/udiscoverit/kosmos/ and the source code will be made publicly available upon publication of the study. The preprint is available on bioRxiv (DOI: 10.64898/2026.08.05.743095).
About the Boyce Thompson Institute (BTI)
As an independent nonprofit research institute affiliated with Cornell University, our scientists are committed to advancing solutions for global food security, agricultural sustainability, and human health. Through groundbreaking research, transformative education, and rapid translation of discoveries into real-world applications, BTI bridges fundamental plant and molecular science with practical impact. Discovery inspired by plants since 1924. Learn more at BTIscience.org.
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